Bacterial CS2 Hydrolases from Acidithiobacillus thiooxidans Strains Are Homologous to the Archaeal Catenane CS2 Hydrolase
نویسندگان
چکیده
منابع مشابه
Bacterial CS2 hydrolases from Acidithiobacillus thiooxidans strains are homologous to the archaeal catenane CS2 hydrolase.
Carbon disulfide (CS(2)) and carbonyl sulfide (COS) are important in the global sulfur cycle, and CS(2) is used as a solvent in the viscose industry. These compounds can be converted by sulfur-oxidizing bacteria, such as Acidithiobacillus thiooxidans species, to carbon dioxide (CO(2)) and hydrogen sulfide (H2S), a property used in industrial biofiltration of CS(2)-polluted airstreams. We report...
متن کاملTransmission electron microscopy enables the reconstruction of the catenane and ring forms of CS2 hydrolase.
Transmission electron microscopic studies on CS2 hydrolase provide direct evidence for the existence of the hexadecameric catenane and octameric ring topologies. Reconstructions of both protein assemblies are in good agreement with crystallographic analyses of the catenane and ring forms of CS2 hydrolase.
متن کاملEvidence that the catenane form of CS2 hydrolase is not an artefact.
CS2 hydrolase, a zinc-dependent enzyme that converts carbon disulfide to carbon dioxide and hydrogen sulfide, exists as a mixture of octameric ring and hexadecameric catenane forms in solution. A combination of size exclusion chromatography, multi-angle laser light scattering, and mass spectrometric analyses revealed that the unusual catenane structure is not an artefact, but a naturally occurr...
متن کاملDiversity and ecophysiology of new isolates of extremely acidophilic CS2-converting Acidithiobacillus strains.
Biofiltration of industrial carbon disulfide (CS2)-contaminated waste air streams results in the acidification of biofilters and therefore reduced performance, high water use, and increased costs. To address these issues, we isolated 16 extremely acidophilic CS2-converting Acidithiobacillus thiooxidans strains that tolerated up to 6% (vol/vol) sulfuric acid. The ecophysiological properties of f...
متن کاملFun in CS2
We report our experience of including the implementation of a player of a computer game as a programming project in a CS2 course focusing in data structures and algorithms. Every semester, an instructor designs the rules of a game, prepares its visualization, and implements an elementary player with a very simple strategy. The game is then delivered to students who, as a first step in order to ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2013
ISSN: 0021-9193
DOI: 10.1128/jb.00627-13